The **t-test** is a statistical test of whether two sample means (averages) or proportions are equal. It was invented by William Sealy Gosset, who wrote under the pseudonym “student” to avoid detection by his employer (the Guinness Brewing Company). Guinness prohibited publications by employees, because another employee had divulged trade secrets in writing.

There are also one-sample versions of a the t-test, to tell if a sample has a mean equal to some fixed value, but these are relatively little used.

**When to use a t-test**

A t-test can be used to compare two means or proportions. The t-test is appropriate when all you want to do is to compare means, and when its assumptions are met (see below). In addition, a t-test is only appropriate when the mean is an appropriate when the means (or proportions) are good measures. See my earlier article for guidance on when to use the mean.

**Matched and unmatched t-tests**

There are two forms of the t-test. In the unmatched t-test, or independent t-test, it is assumed that the two samples are independent. In non-technical language, two samples are independent when knowing something about one does not affect what we know abou the other. For example, the average heights of men and women, drawn randomly from a population, are independent, since knowing the height of a particular man tells us nothing about the height of any particular woman. In a matched t-test, the two sample are not independent; for example, the heights of husbands and wives are not independent, since taller men may be married to taller women. More obviously, the length of people’s right and left feet are dependent, because knowing the size of a right foot tells us a lot about the size of the left foot.

**Assumptions of the t-test**

As noted above, the independent samples t-test assumes the two samples are independent. In addition, both forms of the t-test assume that the variances of the two populations are equal. There are good ways to adjust for unequal variances, provided that the sample sizes of the two

samples are approximately equal. However, if the variances are very different and the sample sizes are different, then the t-test is not a good measure. In addition, as noted above, the t-test only makes sense when the mean makes sense.

**If not the t-test, then what?**

If the t-test is not appropriate, then one alternative is a nonparametric test, such as Wilcoxon’s test. Another alternative is a permutation test, or a bootstrap. In my opinion, all three alternatives ought to be used more often.

**The t-test in SAS**

Suppose one wishes to test if men are heavier than women in a given population. If you sample 5 men and 5 women at random, you might get something like this:

Men: 140 180 188 210 190

Women: 120 190 129 120 130

You could read that into SAS® using

data ttest;

input sex $ weight @@;

datalines;

M 140 F 120 M 180 F 190 M 188 F 129 M 210 F 120 M 190 F 130

;

run;

and then run a t-test by using

proc ttest data = ttest;

class sex;

var weight;

run;

**The t-test in R**

In R, one could read the same data in using

sex <- c(rep(‘M’, 5), rep(‘F’, 5))

weight <- c(140, 180, 188, 210, 190, 120, 190, 129, 120, 130)

and then run a t-test using

t.test(weight~sex)

The output looks like

Welch Two Sample t-test

data: weight by sex

t = -2.4982, df = 7.851, p-value = 0.03758

alternative hypothesis: true difference in means is not equal to 0

95 percent confidence interval:

-84.364290 -3.235710sample estimates:

mean in group F mean in group M

137.8 181.6

Which is terser than the SAS output, but says essentially the same thing. However, by default, R uses the Welch t-test, which does not assume the variances are equal. To get the test with the assumption, you would use

t.test(weight~sex, var.equal = T)

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First, you can’t analyze the effect of anything on anything in an observational study – at best, you can establish association

Second, you probably want a longitudinal design here, with measurements at at least 3 time points on each person. Then you can use a multilevel model to analyze the data.

Dear Sir, Can you please confirm whether I can use t tests in the following situation? To test whether there is a relationship between the education levels of CEOs (bachelors, masters, PhDs ) and firm performance? I know t tests have two groups but there are 4 groups in the case given above. However, I could say that there are only two groups in the case given above if I refer to the groups as terminal degrees and firm performance. I am a bit confused about this, can you provide some expert conclusion? Thank you,

No. You should use regression, not t tests.

As t-test required equal variance assumption, but in practical is it possible to have equal variance of two sample?

Thank you

Aboli

The usual t-test assumes approximately equal variances but there are variations that relax this a bit. Variances are never exactly equal, but they can be close. If the distributions are very dissimilar, you should use a different test.

Hello, I am trying to figure out which statistical test to use for this question: it’s given two sets of data and the question is “do they show a difference in soil depth under the mounds compared to not being under a mound?”

Would i use a t-test to investigate if there’s a difference?

Thank you

Hello, I am trying to figure out which statistical test to use for this question: it’s given two sets of data and the question is “do they show a difference in soil depth under the mounds compared to not being under a mound?”

Would i use a t-test to investigate if there’s a difference?

Thank you

Yes, you could do that.

I am trying to understand when do u sue one tail t-test or when to use two tailed test . please make it simpler to understand .

The simple answer is to never use one tailed.

Hi,

I am comparing several means derived from separate experiments. Height difference in plants grown in light and dark.

I am confused as to what T-test to use. I am going round in circles arguing the different points with myself.

Any help would be appreciated.

Thanks in advance

Hi Liz

This sounds like independent samples t-test, unless the plants in the light and dark were somehow matched to each other.