I wanted to do PERMANOVA analysis to know the difference/variance between my sample groups. But my data is not homogenous (I performed Permutation test for homogeneity of multivariate dispersions test in R) and the replications inside each group is also different. For ex. Three different regions has different sample numbers – >Region A = 36, B = 48 and C = 54. What is the best method to use here? Thanks.

]]>This sounds like independent samples t-test, unless the plants in the light and dark were somehow matched to each other. ]]>

I am comparing several means derived from separate experiments. Height difference in plants grown in light and dark.

I am confused as to what T-test to use. I am going round in circles arguing the different points with myself.

Any help would be appreciated.

Thanks in advance

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